Circulating autoantibodies that react against several beta-cell antigens, i.e. insulin, GAD65, IA-2 and ZnT8 can serve as markers of type 1 diabetes. Since rapid immunodiagnostic tests detecting these autoantibodies from human serum samples are very useful for diagnosis, an antibody capture dipstick lateral flow assay for IA-2 autoantibodies was developed. This immunochromatographic assay uses colloidal gold to visualize the reaction and take 30 min to carry out. Another advantage is the low cost of the dipstick assay, which means that it could be easily used in a clinical setting to obtain quick test results. The sensitivity and specificity of the lateral flow assay is compared to the one of IA-2 radioimmunoassay (RIA) from RSR.